ISO/TS 20224-2:2020
International Standard
Current Edition
·
Approved on
31 July 2020
Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 2: Ovine DNA detection method
ISO/TS 20224-2:2020 Files
English
17 Pages
Current Edition
OMR
48.43
ISO/TS 20224-2:2020 Scope
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep (Ovis aries).
The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene (PRLR) (i.e. GenBank accession number AF041979.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).
Best Sellers
GSO 150-2:2013
Gulf Standard
Expiration dates for food products - Part 2 :
Voluntary expiration dates
OS GSO 150-2:2013
GSO 150-2:2013
Omani Standard
Expiration dates for food products - Part 2 :
Voluntary expiration dates
OS GSO 9:2022
GSO 9:2022
Omani Standard
Labeling of prepackaged food stuffs
GSO 9:2022
Gulf Technical Regulation
Labeling of prepackaged food stuffs
Recently Published
ISO 24131-4:2026
International Standard
Internal protection by polymeric lining for ductile iron pipes — Requirements and test methods — Part 4: Ceramic epoxy lining
ISO 23611-1:2026
International Standard
Soil quality — Sampling of soil invertebrates — Part 1: Hand-sorting and extraction of earthworms
ISO 24829:2026
International Standard
Plastics — Polyether polyols and polymer polyols — Determination of aldehydes and ketones
ISO 12480-5:2026
International Standard
Cranes — Safe use — Part 5: Bridge and gantry cranes