ISO 19820:2016 specifies a method for the determination of the lethal effects of toxicants to Brachionus plicatilis after 24 h or 48 h exposure.

The method is applicable to the following:

a) chemical substances which are soluble or which can be maintained as a stable suspension of dispersions under the conditions of the test;

b) industrial or sewage effluents, treated or untreated, if appropriate after decantation, filtration, or centrifugation;

c) marine or estuarine waters;

d) sediment elutriates/eluates.